Conductive Chloride Flux Across Amphibian Skin - Inhibition By Indacrinone and Cobalt Ion
(1992) Biochimica et Biophysica Acta : international journal of biochemistry and biophysics — Vol. 1104, n° 1, p. 174-178 (1992)
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- Abstract
- When amphibian skin was incubated under conditions in which transepithelial sodium transport was abolished, a conductive transepithelial Cl- flux arose when Cl- was removed from one of the compartments. This flux was matched by short-circuit current and it accounted entirely for transepithelial conductance. Cl- influx was larger than efflux; it was linearly related to the magnitude of transepithelial Cl- concentration difference. When applied to the epithelial surface of the tissue, divalent metal cations such as Co2+, and the ethacrynic acid derivative, indacrinone, reduced rapidly and reversibly both transepithelial Cl- (in)flux and short-circuit current. Frog skin proved to be more sensitive to these inhibitors than toad skin. Further characterization of transepithelial Cl- pathway(s) should benefit from the fact that Cl- across amphibian skin can easily be monitored by the short-circuit current method, and from the availability of agents which inhibit this passive flux rapidly and reversibly.
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Citations
Beaujean, V., & Crabbé, J. (1992). Conductive Chloride Flux Across Amphibian Skin - Inhibition By Indacrinone and Cobalt Ion. Biochimica et Biophysica Acta : international journal of biochemistry and biophysics, 1104(1), 174-178. https://doi.org/10.1016/0005-2736(92)90147-E (Original work published 1992)