Complex stiffness of smooth muscle cytoplasm in the presence of Ca-activated brevin
(1991) Journal of Muscle Research and Cell Motility — Vol. 12, n° 4, p. 333-339 (1991)
Files
No attached file found for this publication.
Details
- Authors
- Abstract
- Brevin, an F-actin severing protein, regulates actin gel-sol transformation in a Ca(2+)-dependent way. Here, we tested its effect on the stiffness of the cytoplasm of skinned smooth muscle, in the absence of actin-myosin interaction (inhibited myosin ATPase). Complex stiffness was measured by imposing sinusoidal stretches and releases at different frequencies (1-50 Hz). In the presence of Ca-activated brevin, the stiffness decreased by about 30%, at all frequencies, from its initial values in Ca-free, relaxing solution. This decrease reflected a fall in both elasticity and viscosity of the cytoplasm. We propose that brevin specifically operates on an actin network in parallel with the contractile apparatus, e.g. on the actin-filamin gel.
- Affiliations
Citations
Gailly, P., Gillis, J.-M., & Capony, J. P. (1991). Complex stiffness of smooth muscle cytoplasm in the presence of Ca-activated brevin. Journal of Muscle Research and Cell Motility, 12(4), 333-339. https://doi.org/10.1007/BF01738588 (Original work published 1991)