QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides.

Galka, Pierre;Jamez, Elisabeth;Joachim, Gilles;Soumillion, Patrice
(2017) PLoS One —

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Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and short overlapping ends. Our method called QuickLib should find many applications in synthetic biology; as an example, we easily prepared genetic libraries of Escherichia coli expressing billions of different backbone cyclic peptides.
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Galka, P., Jamez, E., Joachim, G., & Soumillion, P. (2017). QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides. PLoS One. https://doi.org/10.1371/journal.pone.0175146