Cross-talk mediated by Rgg-peptide cell-cell communication systems from Streptococcus salivarius.

Mignolet, Johann;Vande Capelle, Florence;fontaine, Laetitia;Hols, Pascal
(2015) 8th International Conference on Gram-positive Microorganisms and 18th International Conference on Bacilli — Location: Montecatini terme, Italy (21.June.2015)

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The oral cavity is presumably one of the most challenging environments considering the tremendous and diversified bacterial community that struggle to thrive coupled to constant and multiple external changes. Remarkably, the commensal Streptoccoccus salivarius species is one of the prime colonizers of oral mucosal surfaces that remains predominant in the buccal cavity throughout the human lifespan. Furthermore, several lines of evidence argue in favour of a dampening effect of S. salivarius on its closely-related pathogen and dental caries agent S. mutans. In all likelihood, S. salivarius cells are able to sense the physiological state of S. mutans population in order to provide the ad hoc response and endeavour to restrain the spreading of its competitor. Our preliminary results suggest that S. salivarius is able to recognize a signalling peptide excreted by S. mutans, via the orthologous ComRS cell-cell communication system involved in DNA transformation and production of bacteriocins. We are deciphering S. salivarius molecular mechanisms that allow intra- and inter-species cross-talk in the framework of streptococcal population dynamics. A newly discovered family of pheromone-responsive transcriptional regulators called Rgg is of particular interest as it encompasses the ComRS system. We systematically deleted rgg genes and assessed the role of Rgg proteins for various phenotypes such as natural DNA transformation, predation through bacteriocin production, biofilm formation, and global fitness. Currently, we are assessing the impact of cross-talk between Rgg members in a single S. salivarius strain. Future work will include the investigation of inter-species cross-talk in mixed-species biofilm of S. salivarius and S. mutans strains for a given Rgg system, starting with the better characterized ComRS system.
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Mignolet, J. (2015). Cross-talk mediated by Rgg-peptide cell-cell communication systems from Streptococcus salivarius. 8th International Conference on Gram-positive Microorganisms and 18th International Conference on Bacilli, Montecatini terme, Italy. https://hdl.handle.net/2078.5/180999