Tumor-specific CD8 T cells in a primary human breast carcinoma : a detailed analysis
(2016)
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- Authors
- Supervisors
- Coulie, Pierre
- Abstract
- The clinical efficacy of immunostimulatory antibodies targeting the PD-1 pathway in cancer is likely mediated by CD8 T cells recognizing tumor-specific antigens. Whether breast carcinomas (BCs) are infiltrated by such cells is not known. We wished to obtain evidence for the presence of tumor-specific CD8 T cells in primary BCs. Random sets of CD8 T cell clones were derived from 6 BCs that we screened for recognition of candidate tumor-specific antigenic peptides, which were encoded either by mutated genes or by cancer-germline genes. One of these sets contained 6 different clones that recognized 4 mutant peptides, thus demonstrating that some primary BCs are immunogenic. The corresponding tumor displayed a DNA mismatch repair deficiency associated with a high number of mutations compared to the other 5 tumors. In these 5 tumors, the estimated number of presented mutant antigenic peptides was very low or zero, likely explaining the absence of detectable tumor-specific T cells.
- Affiliations
Citations
Schröder, D. (2016). Tumor-specific CD8 T cells in a primary human breast carcinoma : a detailed analysis. https://hdl.handle.net/2078.5/96410