In Vivo Imaging of S-Layer Nanoarrays on Corynebacterium glutamicum.

Dupres, Vincent;Alsteens, David;Pauwels, Kristof;Dufrêne, Yves
(2009) Langmuir : the A C S journal of surfaces and colloids — Vol. 25, n° 17, p. 9653-9655 (2009)

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Abstract
Crystalline bacterial cell surface layers (S-layers) are monomolecular arrays of (glyco)proteins that have recently produced a wealth of new opportunities in nanotechnology. Whereas the in vitro imaging of isolated S-layers is well established, their direct imaging on live cells remains very challenging. Here we use atomic force microscopy (AFM) to visualize S-layer nanoarrays on living Corynebacterium glutamicum bacteria. We demonstrate the presence of two highly ordered surface layers. The most external layer represents the hexagonal S-layer, and the inner layer displays regular patterns of nanogrooves that could act as a biomolecular template promoting the 2-D assembly of S-layer monomers. These nanoscale analyses open new avenues for understanding the structure of protein monomolecular arrays, which is a crucial challenge in current nanoscience and life science research.
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Dupres, V., Alsteens, D., Pauwels, K., & Dufrêne, Y. (2009). In Vivo Imaging of S-Layer Nanoarrays on Corynebacterium glutamicum. Langmuir : the A C S journal of surfaces and colloids, 25(17), 9653-9655. https://doi.org/10.1021/la902238q (Original work published 2009)