Cavity cuticles that surround the bacterial symbionts residing in the leaf cavities of the aquatic fern Azolla and Azolla leaf cuticle on the epidermis outside the cavity (outer cuticle) were submitted to XPS analysis. Differential charging enabled the signals of the microscopic sample and the support signals to be differentiated. The cavity cuticle has a composition similar to cutin. In comparison with the outer cuticle, it is characterized by a lower concentration of aliphatic chains and a higher concentration of oxygen-containing functions. This has implications in term of solute transport between the leaf and the bacteria. Copyright (C) 2002 John Wiley Sons, Ltd.
Genet, M., Jacques, C., Mozes, N., Van Hove, C., Lejeune, A., & Rouxhet, P. (2002). Use of differential charging to analyse microscopic specimens of Azolla leaves by XPS. Surface and Interface Analysis, 33(7), 601-606. https://doi.org/10.1002/sia.1427 (Original work published 2002)