In order to accurately assess the influence of enzymatic hydrolysis pretreatments on biomass anaerobic conversion, we investigated the assay parameters that influence the measured biogas production rate: inoculum bioactivity; substrate/inoculum ratio; gas / liquid equilibria i;e. presence of residual O2, CO2 & pH equilibria, gas temperature, gas humidity. We were able to master phenomena interfering with biogas production kinetics and not taken into account in most published data. This leads to more accurate and reproducible measurements of biogas production rates. Monitoring the gas metabolites at the hour scale is required to investigate the bioconversion of easily digestible substrates and the influence of substrate hydrolysis pretreatments.
Bindels, F., & Gerin, P. (2012). Investigating the kinetics of biogas production: managing the experimental interferences. Oral presentation, Belgian Energy Research Alliance workshop, Louvain-la-Neuve, Belgium. https://hdl.handle.net/2078.5/163183