Cellular site of glucocorticoid-receptor complex formation.

Levinson, B B;Baxter, J D;Rousseau, G G;Tomkins, G M
(1972) Science (New York, N.Y.) — Vol. 175, n° 18, p. 189-190 (1972)

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Authors
  • Levinson, B B
    Author
  • Baxter, J D
    Author
  • Rousseau, G G
    Author
  • Tomkins, G M
    Author
Abstract
The cellular site of binding of dexamethasone by specific glucocorticoid receptors in cultured hepatoma cells was investigated with the use of certain mercurials. p-Chloromercuribenzene sulfonate and p-chloromercuribenzoate inhibit the binding of steroid by receptors in cell-free extracts, but they allow the steroid-receptor complex to form in whole cells. In contrast, HgCl(2) inhibits binding both in extracts and cells. Since both organic mercury compounds, unlike HgCl(2), do not readily enter intact cells, it appears that the specific steroid binding occurs inside the cell rather than at the cell membrane.
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Levinson, B. B., Baxter, J. D., Rousseau, G. G., & Tomkins, G. M. (1972). Cellular site of glucocorticoid-receptor complex formation. Science (New York, N.Y.), 175(18), 189-190. https://hdl.handle.net/2078.5/32484 (Original work published 1972)