Effects of membrane-active compounds on dexamethasone binding to the glucocorticoid receptor in intact cells.
(1984) Journal of Steroid Biochemistry — Vol. 20, n° 1, p. 37-41 (1984)
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- Abstract
- In intact rat hepatoma (HTC) cells, the binding of dexamethasone to the glucocorticoid receptor is inhibited by the calcium ionophore A23187. This effect involves both nuclear-bound and cytoplasmic receptor. Indirect evidence suggests that the inhibition requires intracellular calcium stores. Exposure of the cells to concanavalin A also inhibits dexamethasone binding. Based on earlier work in cell-free systems, it is proposed that membrane-active agents could modulate glucocorticoid hormone action at the receptor level through fluctuations in intracellular free calcium.
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Rousseau, G., & van Bohemen, C. G. (1984). Effects of membrane-active compounds on dexamethasone binding to the glucocorticoid receptor in intact cells. Journal of Steroid Biochemistry, 20(1), 37-41. https://hdl.handle.net/2078.5/86865 (Original work published 1984)