A transcriptional luxAB reporter fusion responding to fluorene in Sphingomonas sp LB126 and its initial characterisation for whole-cell bioreporter purposes
(2001) Research in Microbiology — Vol. 152, n° 10, p. 849-859 (2001)
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- The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-132 was initially characterised and examined for its potential use as a whole-cell biosensor in the perspective of quantifying fluorene in environmental samples. Activity of the reporter gene as a response to fluorene was detectable after 30 rain and was optimal after 4 h. A linear response to fluorene concentrations within the water solubility range was achieved, with a detection limit of 200 mug per litre. Besides fluorene, L-132 weakly responded to the polycyclic aromatic hydrocarbons phenanthrene and dibenzothiophene, whereas strong responses were obtained with 9-fluorenone, 9-hydroxyfluorene, phthalic acid and protocatechuic acid. The latter four compounds are metabolites formed in course of fluorene degradation, which suggested that a fluorene metabolite rather than fluorene itself was the true inducer of the luxAB fusion in L-132. (C) 2001 Editions scientifiques et medicales Elsevier SAS.
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Bastiaens, L., Springael, D., Dejonghe, W., Wattiau, P., Verachtert, H., & Diels, L. (2001). A transcriptional luxAB reporter fusion responding to fluorene in Sphingomonas sp LB126 and its initial characterisation for whole-cell bioreporter purposes. Research in Microbiology, 152(10), 849-859. https://doi.org/10.1016/S0923-2508(01)01268-2 (Original work published 2001)