Impact of cryopreservation on B cell chronic lymphocytic leukaemia phenotype.
(1999) Journal of Immunological Methods — Vol. 228, n° 1-2, p. 13-21 (1999)
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- BACKGROUND AND OBJECTIVES: Freezing is a practical approach for cell preservation for retrospective studies. The aim of this work was to check the cryopreservation impact on B cell chronic lymphocytic leukaemia phenotype. MATERIAL AND METHODS: Blood samples from 15 CLL patients were analyzed freshly and after freezing at -196 degrees C, without separation, and thawing. Results were compared by Student's paired t-test. RESULTS: The phenotype of fresh CLL cells was as follows: CD19+, CD5+, faint CD20, CD23+/-, weak CD22 and sIg, CD37+, HLA-DR+, FMC7-. After cryopreservation, the percentage of CD5 and CD23 positive cells decreased, whereas HLA-DR positive cells increased moderately. The CLL Matutes's score was modified in 6 cases out of 15 (40%). CONCLUSION: Cryopreservation modifies B cell chronic lymphocytic leukaemia phenotype, by decreasing CD5 and CD23 expression.
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Citations
Deneys, V., Thiry, V., Hougardy, N., Mazzon, A. M., Leveugle, P., & De Bruyère, M. (1999). Impact of cryopreservation on B cell chronic lymphocytic leukaemia phenotype. Journal of Immunological Methods, 228(1-2), 13-21. https://doi.org/10.1016/S0022-1759(99)00089-7 (Original work published 1999)