Characterization of NtWOOLLY and NtRAX2-like, two transcription factors involved in the development of glandular trichomes of Nicotiana tabacum.

Hachez, Charles;Berhin, Alice;Gueuning, Louis
(2022) PLANT GROWTH AND FORM 2022, 2nd international Symposium on Quantitative Plant Morphodynamics — Location: Heidelberg, Germany (12.September.2022)

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Trichomes, the epidermal outgrowths covering most aerial plant tissues are found in a very large number of plant species. They can be glandular or non-glandular. As multicellular structures developing post-embryonically, plant glandular trichomes can be used as a paradigm to tackle basic questions about cell development resulting in the formation of specialized multicellular structures. Such aspects cover the determination of cell fate, developmental control of the cell cycle, division and polarity, cell differentiation, and cell-to-cell signalling. A particularly large gap in our current knowledge about glandular trichome formation and patterning in the leaf epidermis is the identification of regulators of entry into the glandular trichome cell fate and of progression through the pathway. In this context, we focus on the functional characterization of NtWOOLLY, a HD-ZIPIV transcription factor and NtRAX2-like, a R2R3 MYB transcription factor. NtWOOLLY and NtRAX2-like are specifically expressed in the protoderm (developing epidermis) of leaf primordia while their expression is turned off in mature leaves. Interestingly, we found that NtRAX2-like and NtWoolly can physically interact. Our working hypothesis is that NtWOOLLY and NtRAX2-like could heteromerize and that such interaction could promote the development of glandular trichomes. Thorough functional characterization of both genes will be achieved through reverse genetics, biochemistry and -omics analyses in order to infer a model providing an in-depth understanding of their mode of action in tobacco.
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Hachez, C., Berhin, A., & Gueuning, L. (2022). Characterization of NtWOOLLY and NtRAX2-like, two transcription factors involved in the development of glandular trichomes of Nicotiana tabacum. PLANT GROWTH AND FORM 2022, 2nd international Symposium on Quantitative Plant Morphodynamics, Heidelberg, Germany. https://hdl.handle.net/2078.5/247303