Heterogeneous surfaces with organized collagen layers for the control of cell behavior
(2012)
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- Authors
- Supervisors
- Dupont, Christine
- Abstract
- (en) Engineering surfaces that mimic the highly structured environment of cells in tissue, has become a key issue in biomaterials science. However, the effect of nanostructured materials on cell behavior has not well been understood yet. In this context, the aim of this thesis is to better understand the cell-material interactions through elaboration of heterogeneous surfaces for the control and modulation of collagen organization and evaluation of pre-osteoblast behavior. Heterogeneous surfaces were prepared using demixing of polystyrene (PS) and poly(methyl methacrylate) (PMMA) from solutions in different solvents and with different polymer ratios. Substrates with PMMA inclusions in a PS matrix and, conversely, substrates with PS inclusions in a PMMA matrix were elaborated. A range of different collagen organizations was obtained on the designed PS/PMMA films. Inclusions size was revealed as an important parameter in the control of collagen organization. If the PS domains were above 600 nm wide, a heterogeneous distribution of collagen was found, in agreement with observations made on pure polymers (assemblies of a few collagen molecules on PMMA vs bigger assemblies on pure PS). Otherwise, fibrils formed in the PS domains, were longer and thinner compared to the ones observed on pure PS. Pre-osteoblast cultures on bare and collagen-coated PS/PMMA films revealed two main effects: (i) the developed cell area on heterogeneous films is intermediate between the ones observed on each pure polymer, (ii) a higher cell density is always observed on heterogeneous films compared to the corresponding pure polymers. Bio-interfaces with chemical heterogeneities at the submicrometer scale thus trigger particular collagen organizations and cell behaviors, which open perspectives for biomaterials science and tissue engineering.
- Affiliations
Citations
Zuyderhoff, E. (2012). Heterogeneous surfaces with organized collagen layers for the control of cell behavior. https://hdl.handle.net/2078.5/163146