Fructose 2,6-bisphosphate. Hormonal regulation and mechanism of its formation in liver.
(1981) Journal of Biological Chemistry — Vol. 256, n° 17, p. 8900-8903 (1981)
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- Vasopressin, phenylephrine, and A23187 cause an accumulation of fructose 2,6-bisphosphate in hepatocytes from fed rats, but not in Ca2+-depleted hepatocytes from fed rats or in phosphorylase kinase-deficient hepatocytes from (gsd/gsd) rats. The effect of vasopressin and phenylephrine is not found in hepatocytes from overnight-starved rats. Thus, the accumulation of fructose 2,6-bisphosphate by these agents may depend on the stimulation of glycogenolysis and on the resulting accumulation of hexose 6-phosphate. In support of this hypothesis, conditions are described for the enzymatic synthesis of fructose 2,6-bisphosphate from fructose 6-phosphate and Mg-ATP in liver extracts. Half-maximal activity (0.8 nmol/min.g) is obtained with about 60 microM fructose 6-phosphate, and the activity can be separated fom phosphofructokinase by ammonium sulfate fractionation. Treatment of rats or isolated hepatocytes with glucagon results in a 4-5-fold decrease in the maximal activity of this enzyme.
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Hue, L., Blackmore, P. F., & Exton, J. H. (1981). Fructose 2,6-bisphosphate. Hormonal regulation and mechanism of its formation in liver. Journal of Biological Chemistry, 256(17), 8900-8903. https://hdl.handle.net/2078.5/133240 (Original work published 1981)