Probe ligation and real-time detection of KPC, OXA-48, VIM, IMP, and NDM carbapenemase genes
(2013) Diagnostic Microbiology and Infectious Disease — Vol. 76, p. 502-505 (2013)
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- Abstract
- The Check-MDR Carba test (Check-Points, Wageningen, Netherlands), which is based on specific molecular recognition of blaNDM, blaKPC, blaOXA-48, blaVIM, and blaIMP genes by DNA probe ligation and real-time PCR detection, was evaluated on 183 well-characterized Gram-negative rods. Representatives of the 5 gene families were accurately identified (specificities and sensitivities of 100%) within 4.5 hours. This test may be helpful to differentiate carbapenem resistance mediated by carbapenemases from those involving other mechanisms. © 2013 Elsevier Inc. All rights reserved.
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Citations
Cuzon, G., Naas, T., Bogaerts, P., Glupczynski, G., & Nordmann, P. (2013). Probe ligation and real-time detection of KPC, OXA-48, VIM, IMP, and NDM carbapenemase genes. Diagnostic Microbiology and Infectious Disease, 76, 502-505. https://doi.org/10.1016/j.diagmicrobio.2013.05.004 (Original work published 2013)