A flow-through enzyme immunoassay for the screening of fumonisins in maize
(2004) Analytica Chimica Acta — Vol. 523, n° 2, p. 229-235 (2004)
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- Abstract
- The format of an existing flow-through enzyme immunoassay has been optimised for the detection of fumonisin B-1 (FB1) and B-2 (FB2) in maize. The visual detection limit i.e. the smallest mycotoxin concentration resulting in no colour development, is 1000 mug/kg. Assay validation was performed using samples spiked with respectively only FBI and a FB1 + FB2 mixture (ratio 1:1). Results showed that within-day and between-day coefficients of variation ranged from respectively 0.4-10.2% and 1.1-9.0%. Naturally contaminated samples were screened with the developed flow-through method and results were compared with fumonisin contamination values obtained by a validated HPLC method. The assay was demonstrated to be accurate and reliable giving no false compliant and only a low percentage of false non-compliant results. The described method offers a simple, rapid and cost-effective screening tool, thus contributing to a better consumers' health protection. (C) 2004 Elsevier B.V. All rights reserved.
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Citations
Paepens, C., De Saeger, S., Sibanda, L., Barna-Vetro, I., Leglise, I., Van Hove, F., & Van Peteghem, C. (2004). A flow-through enzyme immunoassay for the screening of fumonisins in maize. Analytica Chimica Acta, 523(2), 229-235. https://doi.org/10.1016/j.aca.2004.07.044 (Original work published 2004)