cDNA and protein characterization of human MAGE-10
(1999) International Journal of Cancer — Vol. 82, n° 6, p. 901-907 (1999)
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- MAGE genes are frequently expressed in several types of human malignancy and code for antigens recognized by cytotoxic T lymphocytes. We have previously described a monoclonal antibody (MAb), named 6Cl, that recognizes the MAGE-1 protein and cross-reacts with a 72-kDa protein present in lysates of melanoma cells such as MZ2-MEL. To identify this protein, we have screened an expression library prepared from MZ2-MEL cells. Several clones that encoded a protein recognized by antibody 6Cl contained a sequence identical to that of MAGE-10, another member of the MAGE-A gene family. Full-length MAGE-10 cDNA clones, obtained after screening additional cDNA melanoma libraries, were found to be approximately 2.5 kb in length. In vitro translation and transient transfection experiments indicated that MACE-IO codes for a protein of approximately 72 kDa. This product was recognized by MAb 6CI as well as by a polyclonal serum raised against a MAGE-10 peptide, thus demonstrating its identity with MAGE-10. Analysis of MAGE-10 mRNA by RT-PCR confirmed its presence in testis and placenta but not in other normal tissues. Expression of MAGE-10 in melanoma tumors was found to parallel that of MAGE-1. Western blot analysis with the polyclonal anti-MAGE-10 antibody showed the presence of MAGE-10 in lysates of purified trophoblast cells. Immuno-cytochemistry of cultured melanoma cells indicated that MAGE-10 is a nuclear protein. (C) 1999 Wiley-Liss, Inc.
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Rimoldi, D., Salvi, S., Reed, D., Coulie, P., Jongeneel, V., De Plaen, E., Brasseur, F., Rodriguez, AM., Boon, T., & Cerottini, JC. (1999). cDNA and protein characterization of human MAGE-10. International Journal of Cancer, 82(6), 901-907. https://doi.org/10.1002/(SICI)1097-0215(19990909)82:6<901::AID-IJC21>3.0.CO;2-X (Original work published 1999)