Over-expression of natural and variant human H-chain ferritins in E. coli.

Prozzi, Deborah;Crichton, Robert;Davison, J.
(1988) FEBS Letters — Vol. 234, n° 1, p. 61-64 (1988)

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  • Prozzi, DeborahUCLouvain
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  • Crichton, RobertUCLouvain
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  • Davison, J.
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Abstract
The natural human H-chain ferritin was expressed in E. coli using a multi-copy expression vector containing the lambda pL promoter. A variant H-ferritin, having an altered N-terminus, was also produced. These proteins are overproduced (greater than 30% of the soluble protein), correctly assembled into its 24-subunit shell, and able to bind iron. The identity of the products was confirmed using an antibody specific for H-ferritin.
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Prozzi, D., Crichton, R., & Davison, J. (1988). Over-expression of natural and variant human H-chain ferritins in E. coli. FEBS Letters, 234(1), 61-64. https://doi.org/10.1016/0014-5793(88)81303-6 (Original work published 1988)