GES extended spectrum beta-lactamases in Acinetobacter baumannii in Belgium.
(2010) Antimicrobial Agents and Chemotherapy — Vol. 54, n° 11, p. 4872-4878 (2010)
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- During a PCR-based surveillance study of beta-lactam resistance, 125 multidrug-resistant (MDR) Acinetobacter baumannii isolates were obtained from 18 hospitals in Belgium from January 2008 to December 2009. Nine GES-positive A. baumannii isolates were detected at 6 Belgian hospitals. DNA sequencing of the blaGES genes identified GES-11, GES-12 and a novel variant GES-14 which differs from GES-11 by a single amino acid substitution (Gly170Ser). All index isolates were travel-associated and originated from patients transferred from Turkey (n=2), Egypt (n=2) and Palestinian territories (Gaza) (n=2). A nosocomial outbreak involving three additional patients occurred in a burn unit at a single hospital. No clonal relatedness could be established between the 6 index isolates by PFGE analysis. Three different alleles (the plasmid-located blaGES-11 and blaGES-12 and a likely chromosomal-located novel variant blaGES-14) were detected as part of a class 1 integron also including aac6'Ib and dfrA7 genes. Restriction analysis of plasmids suggests a common origin for the plasmids bearing blaGES-11 and blaGES-12. Cloning of the blaGES genes in E. coli identified GES-14 as hydrolyzing imipenem while GES-12 showed the highest specific activity against ceftazidime. This report highlights the emergence of various blaGES-like genes, especially those conferring carbapenem-resistance in A. baumannii and its importation in Western Europe from Middle Eastern countries.
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Bogaerts, P., Naas, T., El Garch, F., Cuzon, G., Deplano, A., Delaire, T., Huang, T.-D., Lissoir, B., Nordmann, P., & Glupczynski, G. (2010). GES extended spectrum beta-lactamases in Acinetobacter baumannii in Belgium. Antimicrobial Agents and Chemotherapy, 54(11), 4872-4878. https://doi.org/10.1128/AAC.00871-10 (Original work published 2010)