Anticholera Toxin Iga-secreting, Igg-secreting and Igm-secreting Cells in Various Rat Lymphoid-tissues After Repeated Intestinal Or Parenteral Immunizations

Solbreux, PM.;Dive, Charles;Vaerman, JP.
(1990) Immunological Investigations : a journal of molecular and cellular immunology — Vol. 19, n° 5-6, p. 435-451 (1990)

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  • Solbreux, PM.
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  • Dive, CharlesUCLouvain
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  • Vaerman, JP.
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Abstract
Single antibody-secreting spot-forming cells (SFC) of the 3 main isotypes were counted in lymphoid cells from the gut lamina propria (LP), Peyer's patches (PP), mesenteric nodes (MN) and spleen (SP) of rats immunized 2-6 times intraduodenally (ID) or intraperitoneally (IP) with cholera toxin (CT). Responses for all isotypes peaked in all tissues after 4 ID- or IP-immunizations at much larger values than previously reported, and significantly decreased thereafter, except in LP and PP after IP-injections, where IgA- or IgG-SFC, but not IgM-SFC, only appeared or increased after 6 IP-doses. The highest IgA-SFC numbers (17% of tested cells) were in LP after 4 ID-doses. The isotype ration was igA > IgG > IgM in LP and PP after ID-injections, but IgG > IgA > IgM in MN and SP after both ID- and IP-routes. The isotype dispersion was much larger in LP and PP than in MN and SP. Our data show that at least 4 IP CT-doses were required to only elicit a few IgG- and almost no IgA-SFC in LP and PP, outlining the need for intestinal CT-immunizations to induce strong mucosal IgA-SFC responses. We also show good systemic responses elicited by enteral and parenteral routes, and the small contribution of PP in total SFC, particularly after parenteral immunizations.
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Solbreux, PM., Dive, C., & Vaerman, JP. (1990). Anticholera Toxin Iga-secreting, Igg-secreting and Igm-secreting Cells in Various Rat Lymphoid-tissues After Repeated Intestinal Or Parenteral Immunizations. Immunological Investigations : a journal of molecular and cellular immunology, 19(5-6), 435-451. https://doi.org/10.3109/08820139009052971 (Original work published 1990)