Non-invasive water flow imaging in plant roots at cellular resolution

Couvreur, Valentin;Pascut, Flavius;Dietrich, Daniela;Leftley, Nicky;Draye, Xavier;et.al.
(2023) FSPM 2023 — Location: Berlin (26.March.2023)

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Authors
  • Couvreur, Valentinorcid-logoUCLouvain
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  • Pascut, FlaviusOptics & Photonics Research Group, Faculty of Engineering, University of Nottingham, UK
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  • Dietrich, DanielaSchool of Biological Sciences, University of Bristol, UK
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  • Leftley, NickyDivision of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire, UK
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  • Draye, Xavierorcid-logoUCLouvain
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  • et. al.
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Abstract
A key impediment to studying water-related mechanisms in plants is the inability to noninvasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling using MECHA (Couvreur et al., 2018; Pascut et al., 2021), can achieve this goal - monitoring hydrodynamics within living root tissues at cell- and sub-second-scale resolutions. Raman imaging of watertransporting xylem vessels in Arabidopsis thaliana mutant roots reveals faster xylem water transport in endodermal diffusion barrier mutants. Furthermore, transverse line scans across the root suggest water transported via the root xylem does not re-enter outer root tissues nor the surrounding soil when en-route to shoot tissues if endodermal diffusion barriers are intact, thereby separating ‘two water worlds’
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Couvreur, V., Pascut, F., Dietrich, D., Leftley, N., Reyt, G., Draye, X., & et al. (2023). Non-invasive water flow imaging in plant roots at cellular resolution. FSPM 2023, Berlin. https://hdl.handle.net/2078.5/220481