Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratory.

Wauters, Georges;Avesani, Véronique;Charlier, Jacqueline;Janssens, Michèle;Delmée, Michel;et.al.
(2005) Journal of Clinical Microbiology — Vol. 43, n° 6, p. 2624-2628 (2005)

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Authors
  • Wauters, GeorgesUCLouvain
    Author
  • Avesani, VéroniqueUCLouvain
    Author
  • Charlier, JacquelineUCLouvain
    Author
  • Janssens, MichèleUCLouvain
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  • Author
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Abstract
Eighty-six Nocardia strains isolated from clinical samples in Belgium were identified by 16S rRNA gene sequencing. Eighty-three (96%) strains belonged to only six Nocardia species: N. farcinica (38 [44%]), N. nova (19 [22%]), N. cyriacigeorgica (13 [15%]), N. brasiliensis (6 [6.9%]), N. abscessus (5 [5.8%]), and N. paucivorans (2 [2.3%]). A gallery of nine conventional and enzymatic tests was developed for the rapid identification of the most common species isolated during this survey. Pyrrolidonyl aminopeptidase, gamma-glutamyl aminopeptidase, alpha-mannosidase, and alpha-glucosidase were found to be highly discriminating and could be used to develop an identification scheme.
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Citations

Wauters, G., Avesani, V., Charlier, J., Janssens, M., Vaneechoutte, M., & Delmée, M. (2005). Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratory. Journal of Clinical Microbiology, 43(6), 2624-2628. https://doi.org/10.1128/JCM.43.6.2624-2628.2005 (Original work published 2005)