Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid 'real-time' polymerase chain reaction.
(2000) Critical care (London, England) — Vol. 4, n° 4, p. 255-261 (2000)
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- Abstract
- STATEMENT OF FINDINGS: We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 103 colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection to result was less than 1h. RTD-PCR has potential for rapid quantitative detection of pathogens in critical care patients, enabling early and individualized treatment.
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Citations
Pirnay, J. P., De Vos, D., Duinslaeger, L., REPER, P., Vandenvelde, C., Cornelis, P., & Vanderkelen, A. (2000). Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid ‘real-time’ polymerase chain reaction. Critical care (London, England), 4(4), 255-261. https://doi.org/10.1186/cc702 (Original work published 2000)