Marine luciferases: are they really taxon-specific ? A putative luciferase evolved by co-option in an echinoderm lineage
(2014) Luminescence : journal of biological and chemical luminescence — Vol. 29, n° Sppl1, p. 15-16 (2014)
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- (en) The cyclic voltammetry of the earthworm electrode as base staterecorded in 8mL of 0.1M phosphate buffer (pH = 7.5) as electrolytesolution with repetitivefive cycles of the potential from 0.5 to -1.0V and potential sweep rate of 50 mVs. Emission of the light was oc-curred continuously in the sweep towards negative potentials andthe intensity of the emission was higher at more values of negativepotential. First step is oxygen reduction. This step initiates lumines-cence in about E = -0.56 V. Generally it tends to move toward neg-ative values. The second step in the reduction of O2occurs at amore negative potential due to H2O2. This step would occur atabout E = -0.90 V and in this potential, the intensity of the emissionis maximum, after this potential at the return cycling towardpositive potential, the light emission would return to the basevalue. For investigation the effect of addition salts solution toelectrolyte solution on EBL process, 10μLof10-6M salts addedinto electrobioluminescence cell and the effect of each otherof the salts investigated individually. The curve of the cyclicvoltammograms in presence of salts solution almost similarbehavior during input potential programming. For exampleas seen in Fig. P0017:2 for about Fe3+ion, the other ions suchas Cu2+,Ca2+,Fe2+and Zn2+have enhance effect on theelectrobioluminescence output, but Fe2+ion had more effec-tive than the others. Therefore we can determine the ions andhydrogen peroxide by the EBL procedure
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Delroisse, J., Flammang, P., & Mallefet, J. (2014). Marine luciferases: are they really taxon-specific ? A putative luciferase evolved by co-option in an echinoderm lineage. Luminescence : journal of biological and chemical luminescence, 29(Sppl1), 15-16. https://hdl.handle.net/2078.5/55687 (Original work published 2014)