Positive regulation of deoxycytidine kinase activity by phosphorylation of Ser-74 in B-cell chronic lymphocytic leukaemia lymphocytes.

Smal, Caroline;Van Den Neste, Eric;Maerevoet, Marie;Poire, Xavier;Bontemps, Françoise;et.al.
(2007) Cancer Letters — Vol. 253, n° 1, p. 68-73 (2007)

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Abstract
Deoxycytidine kinase (dCK) activates several antileukaemic nucleoside analogues. We have recently reported that the activity of dCK, overexpressed in HEK 293T cells, correlates with its phosphorylation level on Ser-74. Here, we show that dCK from B-cell chronic lymphocytic leukaemia (B-CLL) lymphocytes can be detected by an anti-phospho-Ser-74 antibody and that interindividual variability in dCK activity is related to its phosphorylation level on Ser-74. Moreover, pharmacological intervention modified Ser-74 phosphorylation, in close parallel with changes in dCK activity. These results suggest that activation of dCK via phosphorylation of Ser-74 might constitute a new therapeutic strategy to enhance activation and efficacy of nucleoside analogues.
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Smal, C., Van Den Neste, E., Maerevoet, M., Poire, X., Théate, I., & Bontemps, F. (2007). Positive regulation of deoxycytidine kinase activity by phosphorylation of Ser-74 in B-cell chronic lymphocytic leukaemia lymphocytes. Cancer Letters, 253(1), 68-73. https://doi.org/10.1016/j.canlet.2007.01.013 (Original work published 2007)