Association between mannose-binding lectin gene polymorphisms and necrotizing enterocolitis in preterm infants.
(2012) Journal of Pediatric Gastroenterology and Nutrition — Vol. 55, n° 2, p. 160-165 (2012)
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- Abstract
- OBJECTIVES: The aim of the present study was to evaluate whether polymorphisms of the mannose-binding lectin (MBL-2) gene and MBL serum levels on admission to neonatal intensive care unit are associated with necrotizing enterocolitis (NEC) in preterm infants and to verify MBL expression in NEC bowels. METHODS: In this retrospective cohort study, 107 neonates (41 with NEC and 66 controls) were included. MBL-2 genotyping for the promoter polymorphism -221 and for the exon 1 variant alleles at codons 52, 54, and 57 was performed. MBL levels were determined by enzyme-linked immunosorbent assay in 55 infants. Immunohistochemical staining for MBL expression was performed on bowel specimens. The main study outcome was severe NEC (Bell stages II/III). RESULTS: The -221 Y allele and the MBL-2 YY genotype were more frequent in neonates with severe NEC than in controls (P = 0.04 and P = 0.004, respectively). In the multivariate analysis, the MBL-2 YA/YA genotype was associated with NEC (odds ratio = 3.03, 95% confidence interval 1.13%-8.13%, P = 0.024). Neonates with NEC had MBL level on admission >400 ng/mL more frequently than controls (P = 0.043). Among neonates with severe NEC, the deceased neonates were carriers of high or intermediate producing MBL-2 genotypes (P = 0.035). Finally, MBL was highly expressed in intestinal tissue from infants with NEC. CONCLUSIONS: MBL-2 genotypes associated with high MBL serum levels represent a risk factor for NEC. This finding, together with the MBL expression in bowel tissue, supports a role for MBL in the pathogenesis of NEC.
- Affiliations
Bambino Gesù Children’s Hospital – (Italy) Roma Department of Medical and Surgical Neonatology
Citations
Prencipe, G., Azzari, C., Moriondo, M., Devito, R., Inglese, R., Pezzullo, M., Piersigilli, F., Trucchi, A., De Benedetti, F., & Auriti, C. (2012). Association between mannose-binding lectin gene polymorphisms and necrotizing enterocolitis in preterm infants. Journal of Pediatric Gastroenterology and Nutrition, 55(2), 160-165. https://doi.org/10.1097/MPG.0b013e31824e5f7a (Original work published 2012)