Identification et dissection moléculaire de deux modules de transcription impliqués dans la régulation de gènes Hox
(2003)
Files
Lampe.pdf
Restricted Access - Adobe PDF
- 9.59 MB
Details
- Authors
- Supervisors
- Abstract
- Hox genes encode homeobox transcription factors involved in the antero-posterior patterning of embryos, in the development of the limbs and in some aspects of organogenesis. The understanding of developmental processes involving Hox genes, require to unravel the mechanisms governing their expression. The goal of our work was to analyse two DNA regions (1.25 kb and 2.5 kb) previously known to regulate Hox genes. Here we show that Hoxa1 and Hoxa2 proteins in combination with Pbx1a proteins is able to activate in vitro the expression of a reporter directed by the 1.25 kb region and that this activation relies on a short 10-bp sequence (5'-TGATTGATGA-3') corresponding to a Hox/Pbx binding site (HRE1). Electroporation in chick neural tube shows that the 1.25 kb region is active in rhombomere 4 and those posterior to the fifth rhombomere, and that HRE1 is necessary for that activity. The analysis of the 2.5 kb region has unravelled that its 3' moiety contains another Hox/Pbx cis-regulatory element necessary for Hoxa2 mediated activation of a reporter placed under the control of the 2.5 kb region.
- Affiliations
Citations
Lampe, X. (2003). Identification et dissection moléculaire de deux modules de transcription impliqués dans la régulation de gènes Hox. https://hdl.handle.net/2078.5/97420