An anti-adipocyte monoclonal antibody is cytotoxic to porcine preadipocytes in vitro and depresses the development of pig adipose tissue.

De Clercq, L.;Mourot, J;Genart, C.;Davidts, V;Remacle, Claude;et.al.
(1997) Journal of Animal Science : leading source of new knowledge and perspectives in animal science — Vol. 75, n° 7, p. 1791-1797 (1997)

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  • De Clercq, L.
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  • Mourot, J
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  • Genart, C.
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  • Davidts, V
    Author
  • Remacle, ClaudeUCLouvain
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Abstract
A mouse monoclonal antibody of the IgG2b subclass was raised against porcine adipocyte plasma membranes. This antibody did not cross-react in immunocytofluorescence with any tested cell-type or tissue other than porcine adipocytes. Complement-mediated cytotoxicity was demonstrated in primary cultures of porcine stromal-vascular cells. When the antibody and complement were added to already differentiated cultures, the treatment resulted in elimination of lipid-filled preadipocytes, whereas an early treatment of cultures prevented the appearance of these cells. In vivo, injection of newborn pigs with 1 mg/kg of monoclonal antibody on d 2 and 5 of life produced a more than 20% reduction of subcutaneous and leaf fat lipids at 35 d of age, whereas the lipid content of the longissimus muscle remained unaffected. These results demonstrate that early systemic treatment of pigs with a specific anti-adipocyte antibody reduces the fat mass. In addition to their potential in vivo use, monoclonal antibodies directed against adipose determinants may be useful tools for studying adipocyte lineage.
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De Clercq, L., Mourot, J., Genart, C., Davidts, V., Boone, C., & Remacle, C. (1997). An anti-adipocyte monoclonal antibody is cytotoxic to porcine preadipocytes in vitro and depresses the development of pig adipose tissue. Journal of Animal Science : leading source of new knowledge and perspectives in animal science, 75(7), 1791-1797. https://hdl.handle.net/2078.5/46236 (Original work published 1997)