Sublingual grass pollen immunotherapy is associated with increases in sublingual Foxp3-expressing cells and elevated allergen-specific immunoglobulin G4, immunoglobulin A and serum inhibitory activity for immunoglobulin E-facilitated allergen binding to B cells

Scadding, G. W.;Pilette, Charles;Shamji, M. H.;Jacobson, M. R.;Durham, S. R.;et.al.
(2010) Clinical and Experimental Allergy — Vol. 40, n° 4, p. 598-606 (2010)

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  • Scadding, G. W.
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  • Pilette, CharlesUCLouvain
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  • Shamji, M. H.
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  • Jacobson, M. R.
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  • Durham, S. R.
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Abstract
P>Background The mechanisms of sublingual immunotherapy (SLIT) are less well understood than those of subcutaneous immunotherapy (SCIT). Objectives To determine the effects of grass-pollen SLIT on oral mucosal immune cells, local regulatory cytokines, serum allergen-specific antibody subclasses and B cell IgE-facilitated allergen binding (IgE-FAB). Methods Biopsies from the sublingual mucosa of up to 14 SLIT-treated atopics, nine placebo-treated atopics and eight normal controls were examined for myeloid dendritic cells (mDCs) (CD1c), plasmacytoid dendritic cells (CD303), mast cells (AA1), T cells (CD3) and Foxp3 using immunofluorescence microscopy. IL-10 and TGF-beta mRNA expression were identified by in situ hybridization. Allergen-specific IgG and IgA subclasses and serum inhibitory activity for binding of allergen-IgE complexes to B cells (IgE-FAB) were measured before, during and on the completion of SLIT. Results Foxp3+ cells were increased in the oral epithelium of SLIT- vs. placebo-treated atopics (P=0.04). Greater numbers of subepithelial mDCs were present in placebo-treated, but not in SLIT-treated, atopics compared with normal controls (P=0.05). There were fewer subepithelial mast cells and greater epithelial T cells in SLIT- compared with placebo-treated atopics (P=0.1 for both). IgG(1) and IgG(4) were increased following SLIT (P < 0.001). Peak seasonal IgA(1) and IgA(2) were increased during SLIT (P < 0.05). There was a time-dependent increase in serum inhibitory activity for IgE-FAB in SLIT-treated atopics. Conclusions SLIT with grass pollen extract is associated with increased Foxp3+ cells in the sublingual epithelium and systemic humoral changes as observed previously for SCIT. Cite this as: G. W. Scadding, M. H. Shamji, M. R. Jacobson, D. I. Lee, D. Wilson, M. T. Lima, L. Pitkin, C. Pilette, K. Nouri-Aria and S. R. Durham, Clinical & Experimental Allergy, 2010 (40) 598-606.
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Scadding, G. W., Pilette, C., Shamji, M. H., Jacobson, M. R., Lee, D. I., Wilson, D., Lima, M. T., Pitkin, L., Nouri-Aria, K., & Durham, S. R. (2010). Sublingual grass pollen immunotherapy is associated with increases in sublingual Foxp3-expressing cells and elevated allergen-specific immunoglobulin G4, immunoglobulin A and serum inhibitory activity for immunoglobulin E-facilitated allergen binding to B cells. Clinical and Experimental Allergy, 40(4), 598-606. https://doi.org/10.1111/j.1365-2222.2010.03462.x (Original work published 2010)