Molecular identification of porcine interleukin 10: regulation of expression in a kidney allograft model.

Blancho, G;Gianello, Pierre;Germana, S;Baetscher, M;LeGuern, C;et.al.
(1995) Proceedings of the National Academy of Sciences of the United States of America — Vol. 92, n° 7, p. 2800-2804 (1995)

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Authors
  • Blancho, G
    Author
  • Gianello, PierreUCLouvain
    Author
  • Germana, S
    Author
  • Baetscher, M
    Author
  • LeGuern, C
    Author
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Abstract
Clones encoding porcine interleukin 10 (IL-10) were isolated from a cDNA library produced from phytohemagglutinin-activated pig peripheral blood mononuclear cells. The porcine IL-10 nucleotide sequence was found to be highly homologous to the rat, mouse, and human IL-10 counterparts and to one of the open reading frames from the Epstein-Barr virus. In addition, pig IL-10 caused inhibition of gamma-interferon gene transcription as determined by a bioassay. To investigate the possible immunomodulatory role of IL-10, its expression during the induction of tolerance to kidney allografts by cyclosporin A in miniature swine was also investigated. Delayed expression and higher levels of IL-10 were observed in tolerant animals compared with animals rejecting their allografts. Since tolerance is achieved by a short course of cyclosporin A, we have also studied the in vitro effect of this drug on IL-10 gene transcription in blood mononuclear cells and have found that cyclosporin A inhibits IL-10 gene activation in T cells but does not interfere with IL-10 transcription in lipopolysaccharide-activated cells. These results suggest that the overexpression of IL-10, observed in cell populations infiltrating grafts from tolerant animals, may be a function of monocytes and/or B cells.
Affiliations
  • Harvard Medical SchoolTransplantation Biology Research Center, MGH, Boston

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Blancho, G., Gianello, P., Germana, S., Baetscher, M., Sachs, D. H., & LeGuern, C. (1995). Molecular identification of porcine interleukin 10: regulation of expression in a kidney allograft model. Proceedings of the National Academy of Sciences of the United States of America, 92(7), 2800-2804. https://doi.org/10.1073/pnas.92.7.2800 (Original work published 1995)