Detection of Borrelia-burgdorferi in Biological Samples Using the Polymerase Chain-reaction Assay

Debue, M.;Gautier, Ph. E.;Hackel, C.;Vanelsen, A.;Bollen, A.;et.al.
(1991) Research in Microbiology — Vol. 142, n° 5, p. 565-572 (1991)

Files

pdfdocument.pdf
  • Restricted Access
  • Adobe PDF
  • 624.8 KB
Request a copy

Details

Authors
  • Debue, M.
    Author
  • Gautier, Ph. E.Autre
    Author
  • Hackel, C.
    Author
  • Vanelsen, A.
    Author
  • Bigaignon, GeoffroyUCLouvain
    Author
  • Bollen, A.
    Author
Show more
Abstract
Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene. The last set of primers, OspBpc3/pc4, outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Debue, M., Gautier, Ph. E., Hackel, C., Vanelsen, A., Herzog, A., Bigaignon, G., & Bollen, A. (1991). Detection of Borrelia-burgdorferi in Biological Samples Using the Polymerase Chain-reaction Assay. Research in Microbiology, 142(5), 565-572. https://doi.org/10.1016/0923-2508(91)90189-H (Original work published 1991)