Identification of transcription regulators that control V2 interneuron differentiation during spinal cord development
(2021)
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- Authors
- Supervisors
- Clotman, Frédéric
- Abstract
- During spinal cord development, progenitor domains give rise to multiple neuronal populations and subsets that will, after differentiation, ensure fundamental functions related to locomotion. In this work, we show that Vsx1 and Lhx4 transcription factors control V2 interneuron (IN) and motor neuron (MN) differentiation. We show that Lhx4 is sufficient to induce V2a IN and MN differentiation and suggest that it may be the major LIM factor involved in this process. Lhx4 should be considered for in vitro differentiation of spinal neuronal populations. In addition, using Vsx1│tdTomato lineage tracing, we identified an additional V2 IN population not characterized yet. Finally, by combining the lineage tracing model and RNA sequencing, we potentially discovered new subsets of V2 INs. These results constitute a step forward in the understanding of spinal cord development, but also for the generation of neuronal cells useful in various applications, such as cell therapy.
- Affiliations
Citations
Baudouin, C. (2021). Identification of transcription regulators that control V2 interneuron differentiation during spinal cord development. https://hdl.handle.net/2078.5/112653