Influenza A Virus Infection Induces Viral and Cellular Defective Ribosomal Products Encoded by Alternative Reading Frames.
(2019) Journal of immunology (Baltimore, Md. : 1950) — Vol. 202, n° 12, p. 3370-3380 (2019)
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- Authors
Oveissi, Sara 
Tscharke, David C Duan, Mubing Chen, Weisan
- Abstract
- The importance of antiviral CD8 T cell recognition of alternative reading frame (ARF)-derived peptides is uncertain. In this study, we describe an epitope (NS1-ARF2) present in a predicted 14-residue peptide encoded by the +1 register of NS1 mRNA in the influenza A virus (IAV). NS1-ARF2 elicits a robust, highly functional CD8 T cell response in IAV-infected BALB/c mice. NS1-ARF2 is presented from unspliced NS mRNA, likely from downstream initiation on a Met residue that comprises the P1 position of NS1-ARF2 Derived from a 14-residue peptide with no apparent biological function and negligible impacts on IAV infection, infectivity, and pathogenicity, NS1-ARF2 provides a clear demonstration of how immunosurveillance exploits natural errors in protein translation to provide antiviral immunity. We further show that IAV infection enhances a model cellular ARF translation, which potentially has important implications for virus-induced autoimmunity.
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Citations
Zanker, D. J., Oveissi, S., Tscharke, D. C., Duan, M., Wan, S., Zhang, X., Xiao, K., Mifsud, N. A., Gibbs, J., Izzard, L., Dlugolenski, D., Faou, P., Laurie, K. L., Vigneron, N., Barr, I. G., Stambas, J., Van den Eynde, B., Bennink, J. R., Yewdell, J. W., & Chen, W. (2019). Influenza A Virus Infection Induces Viral and Cellular Defective Ribosomal Products Encoded by Alternative Reading Frames. Journal of immunology (Baltimore, Md. : 1950), 202(12), 3370-3380. https://doi.org/10.4049/jimmunol.1900070 (Original work published 2019)