Polyisobutylcyanoacrylate Nanoparticles As Sustained-release System for Calcitonin
(1995) Journal of Controlled Release — Vol. 33, n° 1, p. 23-30 (1995)
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- Abstract
- The potential of nanoparticles of polyisobutylcyanoacrylate as sustained release for peptide was assessed using calcitonin as a model drug. Calcitonin-loaded nanoparticles were obtained following the addition of the peptide before or after polymerization of isobutylcyanoacrylate (CT-NP and CT/NP, respectively). For both formulations, the percentage of binding of calcitonin to the nanoparticles was more than 95% and the particles had an average size of 150 nm. In vitro studies indicate that the release of calcitonin from CT/NP in saline solution containing esterases resulted from the bioerosion of the polymer. However the peptide was not released from CT-NP in this medium. SDS-PAGE electrophoresis and HPLC also showed that calcitonin is tightly bound to the nanoparticles in the CT-NP formulation, very likely by a covalent binding. After intravenous injection in rats, free calcitonin, CT/NP and CT-NP had the same hypocalcemic activity (at the same dose). Following a subcutaneous injection in rats, the two encapsulated forms of calcitonin showed a more important and more prolonged hypocalcemic effect than free calcitonin. 1 h after injection, calcitonin level was lower after CT-NP injection than CT/NP or free calcitonin injection. However, calcitonin level was sustained for more than 24 h after CT-NP injection.
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Tasset, C., Barette, N., Thysman, S., Ketelslegers, J.-M., Lemoine, D., & Préat, V. (1995). Polyisobutylcyanoacrylate Nanoparticles As Sustained-release System for Calcitonin. Journal of Controlled Release, 33(1), 23-30. https://doi.org/10.1016/0168-3659(94)00060-8 (Original work published 1995)