Comparison of a commercially available rep-PCR system (Diversilab(R)) with PCR-ribotyping for typing of Clostridium difficile strains.
(2011) Journal of Clinical Microbiology — Vol. 49, n° 9, p. 3352-3354 (2011)
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- Authors
- Abstract
- This study compared a rep-PCR method (DiversiLab® system) to PCR-ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR-ribotype 027, different rep-types could be distinguished. Rep-PCR showed a higher discriminatory power than PCR-ribotyping. Nevertheless, this method requires technical skill and visual interpretation of rep-PCR fingerprint patterns may be difficult.
- Affiliations
Université Pierre et Marie Curie, Paris, France ER8 Istituto Superiore di Sanità, Rome, Italy Department of Infectious, Parasitic and Immune-mediated Diseases Université Pierre et Marie Curie, Paris, France ER8
Citations
Eckert, C., Van Broeck, J., Spigaglia, P., Burghoffer, B., Delmée, M., Mastrantonio, P., & Barbut, F. (2011). Comparison of a commercially available rep-PCR system (Diversilab(R)) with PCR-ribotyping for typing of Clostridium difficile strains. Journal of Clinical Microbiology, 49(9), 3352-3354. https://doi.org/10.1128/JCM.00324-11 (Original work published 2011)