Begomoviruses are one of the major constraint for crop production in tropical and subtropical regions. Euphorbia mosaic Venezuela virus (EuMVV) is a begomovirus first reported infecting milkweed (Euphorbia heterophylla) and tomato (Solanum lycopersicum) in Venezuela and displaying mild symptoms of yellowing and curling of leaves (1). In this study seven tomato plants exhibiting begomovirus-like symptoms were collected in a field of Zulia, Venezuela. The samples were analysed by PCR using universal primers for begomoviruses (2). Begomovirus incidence was confirmed for all samples and sequencing of the expected viral DNA fragments revealed the presence of EuMVV in three samples. One of the EuMVV isolates was highly divergent when compared with previous reported isolates (1). In order to characterize this EuMMV isolate (hereafter, Zulia 216) further assays were carried out to perform full-genome amplification and production of an infectious clone.
Romay Gonzalez, G., Chirinos, D. T., Geraud-Pouey, F., Gillis, A., Mahillon, J., Desbiez, C., & Bragard, C. (2016). A new strain of Euphorbia mosaic Venezuela virus displays severe symptoms in tomato planst. 13th International Plant Virus Epidemiology Symposium, Avignon, France. https://hdl.handle.net/2078.5/255780