Role of NTCP in HBV infection of mesenchymal stem cells derived hepatocytes and relevance of its ontogenesis for liver diseases
(2018)
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- Abstract
- The sodium taurocholate cotransporter polypeptide (NTCP), was identified as the first functio- nal receptor for HBV entry. The purpose of the thesis is to i) evaluate the potential role of NTCP in allowing HBV entry and infection of hepatocytes generated after differentiation of umbilical cord mesenchymal stem cells (D-UCMSCs) and ii) appraise its expression during human liver development. In this work, we prove that HBV infection is NTCP-mediated in D-UCMSCs and showed that HBV could modulate the expression of CYP7A1 and CYP3A4 mRNA, but not of NTCP mRNA. As NTCP ontogeny during human development remained largely unexplored, we studied its cellular and tissular expression from fetal to adult stage. Our data from posttrans- lational glycosylation modifications demonstrated that NTCP maturation require at least one year of age. These findings open more questions on how HBV can efficiently infect newborn hepatocytes immediately after birth.
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Sargiacomo, C. (2018). Role of NTCP in HBV infection of mesenchymal stem cells derived hepatocytes and relevance of its ontogenesis for liver diseases. https://hdl.handle.net/2078.5/47332