Crystal structure of the IL-22/IL-22R1 complex and its implications for the IL-22 signaling mechanism

Bleicher, Lucas;de Moura, Patricia Ribeiro;Watanabe, Leandra;Colau, Didier;Polikarpov, Igor;et.al.
(2008) FEBS Letters — Vol. 582, n° 20, p. 2985-2992 (2008)

Files

pdfdocument.pdf
  • Restricted Access
  • Adobe PDF
  • 1.31 MB
Request a copy

Details

Authors
Show more
Abstract
Interleukin-22 (IL-22) is a member of the interleukin-10 cytokine family, which is involved in anti-microbial defenses, tissue damage protection and repair, and acute phase responses. Its signaling mechanism involves the sequential binding of IL-22 to interleukin-22 receptor 1 (IL-22R1), and of this dimer to interleukin-10 receptor 2 (IL-10R2) extracellular domain. We report a 1.9 A crystal structure of the IL-22/IL-22R1 complex, revealing crucial interacting residues at the IL-22/IL-22R1 interface. Functional importance of key residues was confirmed by site-directed mutagenesis and functional studies. Based on the X-ray structure of the binary complex, we discuss a molecular basis of the IL-22/IL-22R1 recognition by IL-10R2.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Bleicher, L., de Moura, P. R., Watanabe, L., Colau, D., Dumoutier, L., Renauld, J.-C., & Polikarpov, I. (2008). Crystal structure of the IL-22/IL-22R1 complex and its implications for the IL-22 signaling mechanism. FEBS Letters, 582(20), 2985-2992. https://doi.org/10.1016/j.febslet.2008.07.046 (Original work published 2008)