Adhesive properties of Staphylococcus epidermidis probed by atomic force microscopy.
(2011) Physical Chemistry Chemical Physics — Vol. 13, n° 21, p. 9995-10003 (2011)
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- Abstract
- Mapping of the surface properties of Staphylococcus epidermidis and of biofilm forming bacteria in general is a key to understand their functions, particularly their adhesive properties. To gain a comprehensive view of the structural and chemical properties of S. epidermidis, four different strains (biofilm positive and biofilm negative strains) were analyzed using in situ atomic force microscopy (AFM). Force measurements performed using bare hydrophilic silicon nitride tips disclosed similar adhesive properties for each strain. However, use of hydrophobic tips showed that hydrophobic forces are not the driving forces for adhesion of the four strains. Rather, the observation of sawtooth force-distance patterns on the surface of biofilm positive strains documents the presence of modular proteins such as Aap that may mediate cell adhesion. Treatment of two biofilm positive strains with two chemical inhibitor compounds leads to a loss of adhesion, suggesting that AFM could be a valuable tool to screen for anti-adhesion molecules.
- Affiliations
DTU Chemistry, Building 207, Technical University of Denmark, DK-2800 Lyngby, Denmark Department of Chemistry DTU Chemistry, Building 207, Technical University of Denmark, DK-2800 Lyngby, Denmark. Department of Chemistry Center for Systems Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark. Department of Systems Biology
Citations
Hu, Y., Ulstrup, J., Zhang, J., Molin, S., & Dupres, V. (2011). Adhesive properties of Staphylococcus epidermidis probed by atomic force microscopy. Physical Chemistry Chemical Physics, 13(21), 9995-10003. https://doi.org/10.1039/C0CP02800B (Original work published 2011)