Protein and chitin nitrogen contents and protein content in Pleurotus ostreatus var columbinus
(1996) Food Chemistry — Vol. 57, n° 2, p. 223-227 (1996)
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- Proteins and chitin were extracted and purified from dried fruitbodies of P. ostreatus var. columbinus grown on wheat straw and grass hay. Water- and sodium chloride-extractable proteins were purified by dialysis and precipitated by 10% TCA. These proteins contained 15.2% nitrogen (Kjeldahl and amino acid methods). The protein/nitrogen ratio of 6.58 was found and recommended as the new conversion factor. Chitin was purified from acid- and alkali-insoluble residue by 2% KMnO4 and 15% NaHSO3. This chitin contained 5.9% nitrogen and its infrared spectrum was similar to that of commercial chitins. The chitinous nitrogen content was about 0.34% dry weight of fruitbodies. The protein content was calculated from the non-chitinous nitrogen content and the factor 6.58. It was 17.1 and 23.5% dry weight of fruitbodies on wheat straw and grass hay, respectively, rather than 18.4 and 24.4% (as total nitrogen x 6.25). Copyright (C) 1996 Elsevier Science Ltd
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Tshinyangu, K., & Hennebert, GL. (1996). Protein and chitin nitrogen contents and protein content in Pleurotus ostreatus var columbinus. Food Chemistry, 57(2), 223-227. https://doi.org/10.1016/0308-8146(95)00202-2 (Original work published 1996)