Activation of muscarinic receptors increases the concentration of free Na+ in mouse pancreatic B-cells

Gilon, Patrick;Henquin, Jean-Claude
(1993) FEBS Letters — Vol. 315, n° 3, p. 353-356 (1993)

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The fluorescent probe SBFI was used to monitor the influence of acetylcholine (ACh) on the cytosolic concentration of free Na+ (Na+i) in single mouse pancreatic B-cells. In the presence of 3 mM glucose and 135 mM extracellular Na+, Na+i averaged 16.6 mM. ACh (100 microM) increased Na+i by approximately 80%. This rise was prevented by atropine, a blocker of muscarinic receptors, and by omission of extracellular Na+, but still occurred if the sodium pump was blocked by ouabain. It was unaffected by tetrodotoxin, a blocker of voltage-sensitive Na+ channels, and was not mimicked by depolarization of the cells with high K+. It is concluded that activation of muscarinic receptors increases the membrane permeability to Na+ in pancreatic B-cells.
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Gilon, P., & Henquin, J.-C. (1993). Activation of muscarinic receptors increases the concentration of free Na+ in mouse pancreatic B-cells. FEBS Letters, 315(3), 353-356. https://doi.org/10.1016/0014-5793(93)81193-4 (Original work published 1993)