The selection of mutant enzymes with novel properties from libraries is emerging as a very powerful strategy for enzyme engineering. The past year has witnessed significant progress on several fronts: new and improved methods have been developed for the creation of libraries and advances have been made in screening and selection techniques. The results achieved demonstrate the enormous potential of the methods and leave questions open for further studies.
Soumillion, P., & Fastrez, J. (2001). Novel concepts for selection of catalytic activity. Current Opinion in Biotechnology, 12(4), 387-394. https://doi.org/10.1016/S0958-1669(00)00232-9 (Original work published 2001)