Role of the endothelin axis in the proliferation of human thyroid cancer cells
(2007) Clinical Endocrinology — Vol. 67, n° 4, p. 552-556 (2007)
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- Objective: Endothelin-1 (ET-1) may play a role in carcinogenesis. ET-1 axis is overexpressed in thyroid carcinoma. We investigated the expression and the production of ET-1 by thyroid cancer cells as well as the effect of ET-1 receptor antagonism on cell proliferation. Design: Human papillary and follicular thyroid carcinoma cell lines were cultured. Measurements: (i) Prepro-ET-1, ET-1 receptors (ETA R and ETB R) and ET-1 converting enzyme (ECE) by reverse transcriptase polymerase chain reaction (RT-PCR); (ii) the presence of ETA R by western blot; (iii) ET-1 concentrations in medium by an enzyme immunometric assay; (iiii) the proliferation of cells by BrdU and tritiated thymidine incorporation. Results: RT-PCR detected the presence of mRNA for prepro-ET-1, ETA R and ECE in papillary and follicular carcinoma cells. ETB R was only expressed by follicular cells. ETA R was also detected in both cell types by western blot. Measurements of ET-1 concentrations demonstrated a secretion of active ET-1 by the cells. ETA R antagonism with atrasentan reduced cell proliferation by 16% in papillary carcinoma cells (P < 0.05) and by 51% in follicular carcinoma cells (P < 0.001). Conclusions: Papillary and follicular carcinoma cells express all components of the ET-1 axis. ET A R antagonism exerts antiproliferative effects, which opens up new therapeutic perspectives in thyroid carcinoma. © 2007 The Authors.
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Donckier, J., Mertens-Strijthagen, J., & Flamion, B. (2007). Role of the endothelin axis in the proliferation of human thyroid cancer cells. Clinical Endocrinology, 67(4), 552-556. https://doi.org/10.1111/j.1365-2265.2007.02923.x (Original work published 2007)