Validation of a method to detect central nervous system acting drugs in blood by gc/ms. application in cases of death caused by phenothiazines in the north of chile
(2014) Journal of the Chilean Chemical Sociaty — Vol. 59, n° 3, p. 2602-2605 (2014)
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- Abstract
- A simple and rapid analytical method is developed and validated for the determination of antiepileptic and antipsychotic drugs in human whole blood using a solid-phase extraction and quantification by gas chromatography-mass spectrometry (GC/MS). Prazepam was used as internal standard (IS). The specificity, linearity, intra- and inter-assay precision and accuracy, and extraction recovery were fully evaluated. The limits of detection and quantification were in the range of 0.52 - 0.98 μg/mL and 1.56 - 2.97 μg/mL for antiepileptic drugs (pentobarbital, phenobarbital and carbamazepine) and 1.31 - 2.94 ng/mL and 3.98 - 8.89 ng/mL for antipsychotic drugs (chlorpromazine and thioridazine), respectively. The relative standard deviation (RSD) was less than 7.07%, while the intra-day accuracy was < 3.21% and the inter-day was < 8.80%, referred to RSD. The developed methodology was applied in forensic cases of death caused by phenothiazines in the North of Chile during 2010-2012.
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Bravo, F., Benites, J., Buc Calderon, P., Briceño, M., Rios, D., Contzen, M. C., Venegas, K., & Ulloa, R. (2014). Validation of a method to detect central nervous system acting drugs in blood by gc/ms. application in cases of death caused by phenothiazines in the north of chile. Journal of the Chilean Chemical Sociaty, 59(3), 2602-2605. https://doi.org/10.4067/S0717-97072014000300016 (Original work published 2014)