Deciphering the role of mitochondrial ROS in radiation therapy through their direct detection in vivo
(2025)
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- Abstract
- Radiotherapy is the second most commonly used therapy against cancer, yet tumor hypoxia remains one of the main mechanisms of resistance. Inhibiting the mitochondrial respiratory chain is a promising strategy in preclinical models, but clinical results remain modest. One hypothesis is that mitochondrial ROS production impacts the response to radiotherapy. Addressing this question requires an in vivo method capable of specifically detecting mitochondrial ROS. In this work, we characterized the ability of electron paramagnetic resonance (EPR) spectroscopy to distinguish mitochondrial ROS from cytosolic and extracellular ROS. We further demonstrated that modulation of mitochondrial ROS at the time of irradiation influences the response to radiotherapy independently of the oxygen effect, establishing mitochondrial redox status as a key determinant of therapeutic response.
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Citations
Mathieu, B. (2025). Deciphering the role of mitochondrial ROS in radiation therapy through their direct detection in vivo.