Presentation of mouse tum- P91A antigen from chimeric proteins with different subcellular localizations by class I molecules of the major histocompatibility complex.
(1993) European journal of immunology — Vol. 23, n° 7, p. 1731-1734 (1993)
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- Abstract
- Like many antigens presented by class I molecules the mouse Ld-binding tum- antigen P91A derives from a cytosolic protein. To decide how stringent this localization is for presentation to cytotoxic lymphocytes (CTL) the P91A template has been inserted in the cDNA of rat esterase ES-10, a protein located in the endoplasmic reticulum (ER), and in the cDNA of mouse interleukin-9, a secretory product of lymphocytes. The esterase construct was also engineered to replace the C-terminal leucine by arginine, which causes secretion of the protein, or to delete the N-terminal presequence, which prevents transfer of the nascent chain to the ER. After cell-free transcription-translation, or transfection in COS cells, the products of the chimeric cDNA had the expected size and localization; however, the truncated form of esterase remained undetected in COS cells. The various chimeric templates were transfected in P1.HTR cells (H-2d); upon challenge with Ld-restricted anti-P91A CTL the cells were lyzed almost as efficiently as cells transfected with the full-length P91A cDNA. We conclude that peptide fragments that bind to class I molecules of the major histocompatibility complex can be generated in the ER.
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Godelaine, D., Van Pel, A., Van Malderen, M., & Beaufay, H. (1993). Presentation of mouse tum- P91A antigen from chimeric proteins with different subcellular localizations by class I molecules of the major histocompatibility complex. European journal of immunology, 23(7), 1731-1734. https://doi.org/10.1002/eji.1830230752 (Original work published 1993)