Assessment of different methods studying the impact of carbon nanomaterials on platelet function
(2012) Immunoanalyse et Biologie Specialisée — Vol. 27, n° 1, p. 41 (2012)
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- Objectives.– We aimed to validate an universal, fast, accurate, reliable and relevant toxicological preclinical screening test to measure the potential impact on primary haemostasis of nanoparticles (NP) whatever their physicochemical properties. Materials and methods.– Four types of carbon nanoparticles (Carbon Black [CB], Fullerenes [C60], Single Wall Carbon Nanotubes [SWCNT], Multi Wall Carbon Nanotubes [MWCNT]) considered as promising in medical applications were investigated. The interference of these nanoparticles on Light Transmission Aggregometry (LTA), Whole-blood Impedance Aggregometry (Multiplate®), Platelet Function Analyzer-100 (PFA-100®) and Impact-R® was studied before the assessment of their effect on platelet function (adhesion, activation and aggregation). Interference and functional impact were also analyzed by transmission and scanning electron microscopy. Results.– Maximal concentrations of C60, CB, SWCNT, MWCNT that may be tested with optical methods like LTA are 500, 10, 500 and 100 μg/mL, respectively. Each nanoparticle interferes by flux obstruction with PFA-100® at concentration higher than 10 μg/mL. Whole-blood impedance aggregometry was not considered as a suitable method because of the interaction between negatively charged nanoparticles and the impedance measures. Impact-R ® showed absence of interference of C60, CB, SWCNT, MWCNT up to 250, 500, 500 and 250 μg/mL, respectively. Furthermore, the addition of Bovine Serum Albumin 7.4 g/L (final concentration) to mimic human blood viscosity abolishes the interference of C60 and MWCNT. Below cut-offs without any interference, none of the nanoparticle has a significant effect on the platelet function, whatever the method used. Conclusion.– Impact-R® is the most adapted test to assess the effect of manufactured NPs on platelet function.
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Mullier, F., Laloy, J., Robert, S., Alpan, L., Mejia, J., Piret, J., BAILLY, N., Lucas, S., Chatelain, B., Toussaint, O., Masereel, B., Rolin, S., & Dogné, J. (2012). Assessment of different methods studying the impact of carbon nanomaterials on platelet function. Immunoanalyse et Biologie Specialisée, 27(1), 41. https://doi.org/10.1016/j.immbio.2011.11.023 (Original work published 2012)