Regulatory T lymphocytes in evaluation of the local protective cellular immune response to Mycobacterium tuberculosis in Romanian patients
(2012) Romanian Biotechnology Letters — Vol. 17, n° 1, p. 6862-6868 (2012)
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- Abstract
- Active suppression by Regulatory T lymphocytes (Treg) might be important in controlling immune responses against Mycobacterium tuberculosis (Mtb). Our aim was to evaluate the local cellular immune response to Mtb, by evaluation of Treg cells in pleural fluid (PF) compared to peripheral blood (PB) from patients with active Mtb infection and healthy Romanian subjects. Tregs were isolated using MACS CD4 +CD25 +CD127 dim/- (Miltenyi) and CD4 CD25 T cells and Foxp3 transcription factor were analyzed by flow cytometry. We found higher % of Tregs in PF compared to PB from patients or healthy Romanian subjects, which might explain the relatively effective local immune response against Mtb infection.
- Affiliations
Stefan S. Nicolau Institute of Virology, Bucharest, Romania Antiviral Therapy Department Carol Davila University of Medicine and Pharmacy, Bucharest, Romania Marius Nasta Institute of Pulmonology Victor Babes Hospital of Infectious and Tropical Diseases, Bucharest, Romania Laboratory of Virology, Immunology and Molecular Biology Elias Emergency University Hospital, Bucharest, Romania Allergology and Clinical Immunology Department
Citations
Diaconu, C.-C., Neagu, A. I., Razvan, L., Gratiela, T., Irina, A., Mihaela, C. E., Coralia, B., Roxana, B. S., Ioana, A., Lilia, M., Laura, D. N., Irina, P., Denisa, D., Stancu, I.-C., Alina, N., Marius, A., & Dragos, B. (2012). Regulatory T lymphocytes in evaluation of the local protective cellular immune response to Mycobacterium tuberculosis in Romanian patients. Romanian Biotechnology Letters, 17(1), 6862-6868. https://hdl.handle.net/2078.5/202755 (Original work published 2012)