Live imaging of mitosomes and hydrogenosomes by HaloTag technology

Martincová, Eva;Voleman, Luboš;Najdrová, Vladimíra;de Napoli, Maximiliano;Doležal, Pavel;et.al.
(2012) PLoS One — Vol. 7, n° 4 (2012)

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  • Martincová, EvaDepartment of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
    Author
  • Voleman, LubošDepartment of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
    Author
  • Najdrová, VladimíraDepartment of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
    Author
  • de Napoli, MaximilianoLaboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Chascomús, Provincia de Buenos Aires, Argentina
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  • Gualdron Lopez, MelisaUCLouvain
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  • Doležal, PavelDepartment of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
    Author
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Abstract
Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes. © 2012 Martincová et al.
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Martincová, E., Voleman, L., Najdrová, V., de Napoli, M., Eshar, S., Gualdron Lopez, M., Hopp, C. S., Sanin, D. E., Tembo, D. L., van Tyne, D., Walker, D., Marcinčiková, M., Tachezy, J., & Doležal, P. (2012). Live imaging of mitosomes and hydrogenosomes by HaloTag technology. PLoS One, 7(4). https://doi.org/10.1371/journal.pone.0036314 (Original work published 2012)